中药化学对照品木蝴蝶苷B的稳定性研究

胡晓茹, 丁倩云, 刘晶晶, 戴忠, 马双成

中国药学杂志 ›› 2020, Vol. 55 ›› Issue (2) : 90-95.

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中国药学杂志 ›› 2020, Vol. 55 ›› Issue (2) : 90-95. DOI: 10.11669/cpj.2020.02.002
论著

中药化学对照品木蝴蝶苷B的稳定性研究

  • 胡晓茹, 丁倩云, 刘晶晶, 戴忠*, 马双成*
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Stability Study of Baicalein-7-O-diglucoside

  • HU Xiao-ru, DING Qian-yun, LIU Jing-jing, DAI Zhong*, MA Shuang-cheng*
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摘要

目的 对木蝴蝶苷B固体和在不同溶液中的稳定性进行研究。方法 采用HPLC-DAD对不同温度下的木蝴蝶苷B固体和不同条件下的木蝴蝶苷B溶液进行色谱分离,经过归一化纯度分析对其稳定性进行比较和分析,明确木蝴蝶苷B的稳定性影响因素;然后采用HPLC-MS对木蝴蝶苷B在不同溶剂中主要降解产物进行分析。结果 ①木蝴蝶苷B固体在40、80和105 ℃下稳定、且对光稳定;②木蝴蝶B在水中较为稳定,在体积分数50%、70%甲醇、体积分数50%、70%乙醇溶液中不稳定。③木蝴蝶苷B在体积分数50%甲醇、体积分数50%乙醇和水溶液中降解产物分别为7-甲氧基黄芩素、7-乙氧基黄芩素和黄芩素。结论 本次实验表明,木蝴蝶苷B对热和光稳定,但在溶液状态下不够稳定,尤其在体积分数50%甲醇溶液和体积分数50%乙醇溶液中易发生降解,建议木蝴蝶苷B对照品溶液选择采用水作为溶剂,或者临用现配,并在配制后立即放入冰箱冷藏保存。

Abstract

OBJECTIVE To investigate the stability of baicalein-7-O-diglucoside. METHODS The chromatographic purity of baicalein-7-O-diglucoside and its solution in different condition was analyzed by HPLC-DAD. The degradation products of baicalein-7-O-diglucoside in its solvent were identified by LC-MS. Three degradation products in the solvent of baicalein-7-O-diglucoside were identified with applying electrospray ionization(ESI) source in the positive ion mode.RESULTS ① Baicalein-7-O-diglucoside was stable with light and in 40, 80 and 105 ℃ within the inspection time in solid state. ②Baicalein-7-O-diglucoside in water was stable in room temperature and degrades quickly in 50%methanol and 50% ethanol. ③The degradation products of baicalein-7-O-diglucoside in solvent were 7-methoxy baicalein, 7-ethoxy baicalein and baicalein. The above research provides that baicalein-7-O-diglucoside is stable in solid state. Baicalein-7-O-diglucoside degrades in 50% methanol and 50% ethanol. CONCLUSION Water should be used as the solvent for solution of baicalein-7-O-diglucoside. The solution of baicalein-7-O-diglucoside should be made up before use or stored in the refrigerator immediately after preparation.

关键词

木蝴蝶苷B / 稳定性 / 黄芩素 / 7-甲氧基黄芩素

Key words

baicalein-7-O-diglucoside / stability / baicalein / 7-methoxy baicalein

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导出引用
胡晓茹, 丁倩云, 刘晶晶, 戴忠, 马双成. 中药化学对照品木蝴蝶苷B的稳定性研究[J]. 中国药学杂志, 2020, 55(2): 90-95 https://doi.org/10.11669/cpj.2020.02.002
HU Xiao-ru, DING Qian-yun, LIU Jing-jing, DAI Zhong, MA Shuang-cheng. Stability Study of Baicalein-7-O-diglucoside[J]. Chinese Pharmaceutical Journal, 2020, 55(2): 90-95 https://doi.org/10.11669/cpj.2020.02.002
中图分类号: R284    R917   

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